Zebrafish Whole Mount High-Resolution Double Fluorescent <em>In Situ</em> Hybridization
نویسندگان
چکیده
منابع مشابه
Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
Whole mount in situ hybridization is one of the most widely used techniques in developmental biology. Here, we present a high-resolution double fluorescent in situ hybridization protocol for analyzing the precise expression pattern of a single gene and for determining the overlap of the expression domains of two genes. The protocol is a modified version of the standard in situ hybridization usi...
متن کاملDouble labeling for whole-mount in situ hybridization in mouse.
1.Brugal, G. 1995. Quantitative microscopy and tumor cell proliferation. Bull. Cancer 511S517S. 2.Doyle, A., J.B. Griffiths and D.G. Newell. 1993. Cell and Tissue Culture: Laboratory Procedures. John Wiley & Sons International, Chichester, UK. 3.Gerdes, J., U. Schwab, H. Lemke and H. Stein. 1983. Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cel...
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The photostability and narrow emission spectra of nanometer-scale semiconductor crystallites (QDs) make them desirable candidates for whole-mount fluorescent in situ hybridization to detect mRNA transcripts in morphologically preserved intact embryos. We describe a method for direct QD labeling of modified oligonucleotide probes through streptavidin-biotin and antibody-mediated interactions (an...
متن کاملVisualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
In recent years, the zebrafish embryo has emerged as a popular model to study developmental biology due to traits such as ex utero embryo development and optical transparency. In particular, the zebrafish embryo has become an important organism to study vertebrate kidney organogenesis as well as multiciliated cell (MCC) development. To visualize MCCs in the embryonic zebrafish kidney, we have d...
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In this study, we cloned the myeloperoxidase (mpo) gene of zebrafish and prepared a digoxigenin-labeled mpo RNA probe to investigate mpo gene expression in zebrafish during embryonic development by whole-mount in situ hybridization (WISH). The earliest mpo expression was detected in cells of the intermediate cell mass (ICM) at 18 h post-fertilization (hpf). It was detected 1 to 2 h later in cel...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2009
ISSN: 1940-087X
DOI: 10.3791/1229